![]() Therefore, using insulin for human semen seems to could be suggested for future clinical applications.Īrtificial Insemination (AI) in swines is a rising technique that has been used during the last years at the swine production systems, because it furnish several advantages to the producers just like boar high efficiency, better breeding control and easiness in introducing foreign genetic materials. Based on our findings adding insulin to semen leads to protecting effects against cryopreservation damages and increases sperms motility. Also, the number of motile sperms increased in all insulin groups but it wasn't meaningful (P˃0.05). Insulin at 1000 (ng/ml) decreased DNA fragmentation, significantly (P˂0.05). Mitochondrial ROS also decreased by adding insulin in comparison to the control group, although unmeaningfully (P˃0.05). Results showed that insulin at all doses significantly decreased cytosolic ROS especially in 10 ng/ml group (P˂0.05). Data were analyzed by SPSS software using one-way ANOVA. Then, after thawing sperm motility cytosolic/mitochondrial reactive oxygen species (ROS) levels and DNA fragmentation were analyzed. Samples were cryopreserved for 2 weeks in liquid nitrogen. Cryopreservation of sperms was conducted along with adding 10, 100, 5 (ng/ml) insulin and a control group was also considered by adding distilled water. Semen samples were obtained from 15 normozoospermic men at age 25–40 years of old through masturbation. The aim of this study was to assess the effect of insulin as a prosurvival factor on the most important functional parameters of human spermatozoa during cryopreservation. In conclusion, the addition of 3 IU mL-1 insulin to ram semen extender improved the quality of sperm motility after cooling.Ĭryopreservation of sperms is common therapy but with multiple damages to sperms. There was an increased percentage of progressive sperm motility (%), straightness (%), linearity (%) and beat caudal frequency (Hz) in the insulin group after 24 and 48 hours of cooling (p 0.05). The semen analysis was performed after 24 or 48 hours of cooling. The samples were evaluated for acrosomal integrity by fluorescein using isothiocyanate combined with peanut agglutinin (FITC-PNA) and membrane functionality by the hypoosmotic swelling test. Subsequently, the sperm cells were evaluated for motility and kinetics using computer-assisted semen analysis. The ejaculates were divided into two aliquots with (insulin group) or without (control group) insulin (3 IU mL-1) in the semen extender, and then cooled at 5☌ for 48 hours. Sperm samples were collected from six healthy, mature Santa Inês rams. Therefore, the objective of the current study was to evaluate the effect of insulin addition on ram sperm maintained at 5✬. However, the effects of insulin on the viability of cooled ram sperm are not fully understood. All rights reserved.Insulin is present in the seminal plasma and is involved in sperm activities like motility and capacitation. The sensitivity is sufficient to detect deviations from correct extender preparation before negative effects on sperm quality occur.īoar Extender preparation Quality assurance Semen preservation Semen quality.Ĭopyright © 2014 Elsevier B.V. The refractive-index is an indicator of osmolality and may be used to verify semen extender preparation. Boar sperm quality is affected by inexact extender preparation. The mean percentage of PI negative spermatozoa with active mitochondria on d2 showed a significant difference using ≤60% EC (P=0.016) and ≥140% EC (P<0.001) compared to 100% EC, respectively. Percentages of spermatozoa with intact membranes on d2 resulted in a significant decrease using 50% EC (P<0.001) and ≥150% EC (P=0.005), respectively. An increased number of coiled tails were observed using ≤60% EC (P<0.001). Secondary apical ridge defects were significantly higher using 50% EC (P<0.001) and ≥150% EC (P=0.032) compared to 100% EC, respectively. The percentage of motile spermatozoa in a thermoresistance test on d2 showed a significant drop using ≤70% EC (P=0.047) and ≥140% EC (P=0.004). Total sperm motility with 100% EC differed significantly from ≤70% EC (P<0.001) and 200% EC (P<0.001) on day 1 (d1) and d4, respectively. The refractive index for the correctly prepared extender was 4.6☐.0☋x, corresponded to 316☑6mOsmkg(-1), and correlated highly with osmolality (r=0.99 P<0.001). Twelve boar ejaculates were evaluated for semen quality. For this the refractive index and osmolality of BTS extender concentrations (EC) were recorded in 10%-steps from 50% to 150% and 200% of the correct amount. ![]() A study was performed to see if refractometry can be used as a new quality control tool for boar semen extenders.
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